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Purpose(Empty Backbone) BglFusion compatible integration vector for Streptococcus pneumoniae
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61046 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepPEP1
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSpectinomycin selection for both E. coli and S. pneumoniae. Low copy number p15 origin. Can be used for BglFusion cloning.
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Copy numberLow Copy
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPEP1 was a gift from Jan-Willem Veening (Addgene plasmid # 61046 ; http://n2t.net/addgene:61046 ; RRID:Addgene_61046) -
For your References section:
Gene Expression Platform for Synthetic Biology in the Human Pathogen Streptococcus pneumoniae. Sorg RA, Kuipers OP, Veening JW. ACS Synth Biol. 2014 Jun 4. 10.1021/sb500229s PubMed 24845455
