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pDONRG_P4-P1R:MUM4_0.3Pro_35S
(Plasmid #128558)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 128558 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pDONRG_P4-P1R
  • Backbone manufacturer
    Invitrogen, modified by 10.5511/plantbiotechnology.11.0124a
  • Backbone size (bp) 5892
  • Modifications to backbone
    151 bp within the attR1 sequence there is a thymine to cysteine modification, which does not impact the gateway reaction.
  • Vector type
    Gateway promoter entry clone
  • Promoter MUM4 core promoter with 35S enhancer

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamycin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For selection use 7 ug/ml Gentamicin
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer 5'-GTAAAACGACGGCCAG-3`
  • 3′ sequencing primer 5'-GTAACATCAGAGATTTTGAGACA-3`
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use with Three-way Multi-site Gateway Cloning (Invitrogen).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONRG_P4-P1R:MUM4_0.3Pro_35S was a gift from George Haughn (Addgene plasmid # 128558 ; http://n2t.net/addgene:128558 ; RRID:Addgene_128558)
  • For your References section:

    Identification of a seed coat-specific promoter fragment from the Arabidopsis MUCILAGE-MODIFIED4 gene. Dean GH, Jin Z, Shi L, Esfandiari E, McGee R, Nabata K, Lee T, Kunst L, Western TL, Haughn GW. Plant Mol Biol. 2017 Sep;95(1-2):33-50. doi: 10.1007/s11103-017-0631-7. Epub 2017 Jul 20. 10.1007/s11103-017-0631-7 PubMed 28730525