Purpose(Empty Backbone) Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloning
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||128558||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
* Login to view industry pricing.
Backbone manufacturerInvitrogen, modified by 10.5511/plantbiotechnology.11.0124a
- Backbone size (bp) 5892
Modifications to backbone151 bp within the attR1 sequence there is a thymine to cysteine modification, which does not impact the gateway reaction.
Vector typeGateway promoter entry clone
- Promoter MUM4 core promoter with 35S enhancer
Growth in Bacteria
Bacterial Resistance(s)Gentamycin, 10 μg/mL
Growth instructionsFor selection use 7 ug/ml Gentamicin
Copy numberHigh Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer 5'-GTAAAACGACGGCCAG-3`
- 3′ sequencing primer 5'-GTAACATCAGAGATTTTGAGACA-3` (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
For use with Three-way Multi-site Gateway Cloning (Invitrogen).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONRG_P4-P1R:MUM4_0.3Pro_35S was a gift from George Haughn (Addgene plasmid # 128558 ; http://n2t.net/addgene:128558 ; RRID:Addgene_128558)
For your References section:Identification of a seed coat-specific promoter fragment from the Arabidopsis MUCILAGE-MODIFIED4 gene. Dean GH, Jin Z, Shi L, Esfandiari E, McGee R, Nabata K, Lee T, Kunst L, Western TL, Haughn GW. Plant Mol Biol. 2017 Sep;95(1-2):33-50. doi: 10.1007/s11103-017-0631-7. Epub 2017 Jul 20. 10.1007/s11103-017-0631-7 PubMed 28730525