PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||129100||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)708
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
To design forward PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) ATGGTGAGTAAAGGCGAGGA-3'. To design reverse PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) GAATTCGAGCTCGTTTAAAC-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFA6a-mNeonGreen-HIS3MX6 was a gift from Wei-Lih Lee (Addgene plasmid # 129100 ; http://n2t.net/addgene:129100 ; RRID:Addgene_129100)