Purpose(Empty Backbone) Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||129271||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Modifications to backbonepemI/pemK plasmid stability factor Gateway cloning cassette constitutive promoter
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Gentamicin, 10 μg/mL
Growth Strain(s)ccdB Survival
Copy numberLow Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer see supplemental info for primer sequence
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBBR5pemIKpKan was a gift from Lindsey Burbank (Addgene plasmid # 129271 ; http://n2t.net/addgene:129271 ; RRID:Addgene_129271)
For your References section:Broad-Host-Range Plasmids for Constitutive and Inducible Gene Expression in the Absence of Antibiotic Selection. Burbank L, Wei W. Microbiol Resour Announc. 2019 Sep 5;8(36). pii: 8/36/e00769-19. doi: 10.1128/MRA.00769-19. 10.1128/MRA.00769-19 PubMed 31488530