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pBBR5pemIKpBAD
(Plasmid #129273)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 129273 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBBR1-MCS5
  • Modifications to backbone
    pemI/pemK plasmid stability factor Gateway cloning cassette arabinose-inducible promoter
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer see supplemental info for primer sequence
  • 3′ sequencing primer M13 rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

There is a single mutation in araC- I184V. Depositor confirms this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBBR5pemIKpBAD was a gift from Lindsey Burbank (Addgene plasmid # 129273 ; http://n2t.net/addgene:129273 ; RRID:Addgene_129273)
  • For your References section:

    Broad-Host-Range Plasmids for Constitutive and Inducible Gene Expression in the Absence of Antibiotic Selection. Burbank L, Wei W. Microbiol Resour Announc. 2019 Sep 5;8(36). pii: 8/36/e00769-19. doi: 10.1128/MRA.00769-19. 10.1128/MRA.00769-19 PubMed 31488530