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(Plasmid #129570)


Item Catalog # Description Quantity Price (USD)
Plasmid 129570 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 4969
  • Modifications to backbone
    3xFLAG tag inserted at BamHI site of pGEX-4T-1, regenerating the BamHI site downstream of 3xFLAG, with a unique HindIII site added between the BamHI and EcoRI sites of pGEX-4T-1.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor uses BL21 for protein production.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1-3xFLAG was a gift from Kevin Janes (Addgene plasmid # 129570 ; ; RRID:Addgene_129570)
  • For your References section:

    An ultrasensitive fiveplex activity assay for cellular kinases. Smolko CM, Janes KA. Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8. 10.1038/s41598-019-55998-8 PubMed 31857650