An ultrasensitive fiveplex activity assay for cellular kinases.
Smolko CM, Janes KA
Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129226 | pGEX-4T-1-3xHA-IκBα(1-62) | Encodes HA tagged human IκBα(1-62) for purification by GST affinity |
| 129227 | pET-29b-3xGluGlu-c-jun-His6 | Encodes 3xGluGlu tagged human c-Jun for purification by nickel affinity |
| 129228 | pGEX-4T-1-3xMyc-ERK2(K52R) | Encodes Myc tagged human ERK2(K52R) for purification by GST affinity |
| 129229 | pGEX-4T-1-3xFLAG-HSP27 | Encodes FLAG tagged human HSP27 for purification by GST affinity |
| 129567 | pGEX-4T-1-3xHA | Empty backbone for HA tag |
| 129568 | pGEX-4T-1-3xVSVG | Empty backbone for VSVG tag |
| 129569 | pGEX-4T-1-3xMyc | Empty backbone for Myc Tag |
| 129570 | pGEX-4T-1-3xFLAG | Empty backbone for FLAG tag |
| 129571 | pGEX-4T-1-3xGluGlu | Empty backbone for GluGlu tag |
| 129572 | pGEX-4T-1-3xAU1 | Empty backbone for AU1 tag |
| 129582 | pGEX-4T-1-3xVSVG-GSK3(1-97) | Encodes VSVG tagged GSK3(1-97) for purification by GST affinity |
| 129583 | pET-29b-3xVSVG-GSK3(1-97) | Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity |