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Addgene
An ultrasensitive fiveplex activity assay for cellular kinases.
Smolko CM, Janes KA
Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
129226pGEX-4T-1-3xHA-IκBα(1-62)Encodes HA tagged human IκBα(1-62) for purification by GST affinity
129227pET-29b-3xGluGlu-c-jun-His6Encodes 3xGluGlu tagged human c-Jun for purification by nickel affinity
129228pGEX-4T-1-3xMyc-ERK2(K52R)Encodes Myc tagged human ERK2(K52R) for purification by GST affinity
129229pGEX-4T-1-3xFLAG-HSP27Encodes FLAG tagged human HSP27 for purification by GST affinity
129567pGEX-4T-1-3xHAEmpty backbone for HA tag
129568pGEX-4T-1-3xVSVGEmpty backbone for VSVG tag
129569pGEX-4T-1-3xMycEmpty backbone for Myc Tag
129570pGEX-4T-1-3xFLAGEmpty backbone for FLAG tag
129571pGEX-4T-1-3xGluGluEmpty backbone for GluGlu tag
129572pGEX-4T-1-3xAU1Empty backbone for AU1 tag
129582pGEX-4T-1-3xVSVG-GSK3(1-97)Encodes VSVG tagged GSK3(1-97) for purification by GST affinity
129583pET-29b-3xVSVG-GSK3(1-97)Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity

Antibodies from Article