pHis APEX2-eIF4A1
(Plasmid
#129645)
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PurposeExpress APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHis
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeukaryotic translation initiation factor 4A1
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SpeciesH. sapiens (human)
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GenBank IDNM_001416.4
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Entrez GeneEIF4A1 (a.k.a. DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I)
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Tags
/ Fusion Proteins
- APEX2 (N terminal on backbone)
- 6xHis (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHis APEX2-eIF4A1 was a gift from Nicholas Ingolia (Addgene plasmid # 129645 ; http://n2t.net/addgene:129645 ; RRID:Addgene_129645) -
For your References section:
Proximity RNA Labeling by APEX-Seq Reveals the Organization of Translation Initiation Complexes and Repressive RNA Granules. Padron A, Iwasaki S, Ingolia NT. Mol Cell. 2019 Aug 22;75(4):875-887.e5. doi: 10.1016/j.molcel.2019.07.030. 10.1016/j.molcel.2019.07.030 PubMed 31442426
