rpoDs366C
(Plasmid
#129688)
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PurposeExpression of Sigma 70 S366C
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerpoD
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Alt nameSigma 70
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SpeciesE. coli
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MutationC132S C291S C295S S366C
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Entrez GenerpoD (a.k.a. b3067, ECK3057, alt)
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Tag
/ Fusion Protein
- His6/thrombin (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS found a N149D amino acid variation in the rpoD translation, that the depositing laboratory confirms to be a naturally occurring sequence variant. This will not adversely affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/2020.02.17.953182v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
rpoDs366C was a gift from Jeff Gelles (Addgene plasmid # 129688 ; http://n2t.net/addgene:129688 ; RRID:Addgene_129688) -
For your References section:
Mechanism of upstream promoter element stimulation of transcription at a ribosomal RNA promoter determined by single-molecule imaging. Mumm JP, Friedman LJ, Gelles J. bioRxiv 2020 10.1101/2020.02.17.953182
Map uploaded by the depositor.
