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pFLIPglu-3.2mDelta13
(Plasmid #12992)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRT100/pPZP312
  • Vector type
    Plant Expression ; Plant Nanosensor
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FLIPglu F16A S112A Delta13
  • Alt name
    ECFP-mglB-EYFP
  • Alt name
    Periplasmic glucose binding protein
  • Species
    E. coli
  • Insert Size (bp)
    2280
  • Mutation
    F16A, S112A in binding protein
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PCR of MglB

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLIPglu-3.2mDelta13 was a gift from Wolf Frommer (Addgene plasmid # 12992 ; http://n2t.net/addgene:12992 ; RRID:Addgene_12992)

  • For your References section:

    Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing Mutants. Deuschle K, Chaudhuri B, Okumoto S, Lager I, Lalonde S, Frommer WB. Plant Cell. 2006 Sep . 18(9):2314-25. 10.1105/tpc.106.044073 PubMed 16935985
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