pETMutS-E41A
(Plasmid
#13048)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13048 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET3a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4640
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMutS
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SpeciesThermus aquaticus
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Insert Size (bp)3000
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Mutationmutation Glu41 to Ala full-length
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GenBank IDU33117
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETMutS-E41A was a gift from Peggy Hsieh (Addgene plasmid # 13048 ; http://n2t.net/addgene:13048 ; RRID:Addgene_13048) -
For your References section:
The Phe-X-Glu DNA binding motif of MutS. The role of hydrogen bonding in mismatch recognition. Schofield MJ, Brownewell FE, Nayak S, Du C, Kool ET, Hsieh P. J Biol Chem. 2001 Dec 7. 276(49):45505-8. 10.1074/jbc.C100449200 PubMed 11602569