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pET51b(+)_SNAP-p24
(Plasmid #130718)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 130718 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET51-b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5218
  • Total vector size (bp) 6433
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SNAP-Tag-p24
  • Alt name
    P24 HIV Capsid protein fused to SNAP-Tag
  • Species
    Synthetic
  • Insert Size (bp)
    1371
  • Mutation
    Deleted first Met, Ala and Last Ser from the Enhancer nanobody
  • Tags / Fusion Proteins
    • Hisx10 (C terminal on insert)
    • StrepTag (N terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET51b(+)_SNAP-p24 was a gift from Kai Johnsson (Addgene plasmid # 130718 ; http://n2t.net/addgene:130718 ; RRID:Addgene_130718)
  • For your References section:

    Chemogenetic Control of Nanobodies. Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K. Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7. 10.1038/s41592-020-0746-7 PubMed 32066961