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Depositing Lab
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Depositing OrganizationScripps California (The Scripps Research Institute)
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13096 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 13096-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 13096-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFPC1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3941
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATG5-K130R
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Alt nameATG5
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SpeciesM. musculus (mouse)
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Insert Size (bp)1576
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MutationK130R
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GenBank IDAB048349
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Entrez GeneAtg5 (a.k.a. 2010107M05Rik, 3110067M24Rik, Apg5l, Atg5l, Paddy)
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe fusion marker mCherry is from Roger Tsien at UCSD
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
for mCherry marker protein
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82
DNA (Catalog # 13096-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 13096-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCherry-ATG5-K130R was a gift from Roberta Gottlieb (Addgene plasmid # 13096 ; http://n2t.net/addgene:13096 ; RRID:Addgene_13096) -
For your References section:
Response to myocardial ischemia/reperfusion injury involves Bnip3 and autophagy. Hamacher-Brady A, Brady NR, Logue SE, Sayen MR, Jinno M, Kirshenbaum LA, Gottlieb RA, Gustafsson AB. Cell Death Differ. 2007 Jan;14(1):146-57. doi: 10.1038/sj.cdd.4401936. Epub 2006 Apr 28. 10.1038/sj.cdd.4401936 PubMed 16645637
