PurposeDouble floxed ChRmine-eYFP under the control of Ef1a promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||130996||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Promoter Ef1a
/ Fusion Protein
- eYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer GTTAGGCCAGCTTGGCACTTG
- 3′ sequencing primer GAATACCAGTCAATCTTTCAC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid has a 3'UTR that increases the packaging size to 5.4 kb, which may not be suitable for some AAV capsids
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-Ef1a-DIO ChRmine-eYFP-WPRE was a gift from Karl Deisseroth (Addgene plasmid # 130996 ; http://n2t.net/addgene:130996 ; RRID:Addgene_130996)
For your References section:Cortical layer-specific critical dynamics triggering perception. Marshel JH, Kim YS, Machado TA, Quirin S, Benson B, Kadmon J, Raja C, Chibukhchyan A, Ramakrishnan C, Inoue M, Shane JC, McKnight DJ, Yoshizawa S, Kato HE, Ganguli S, Deisseroth K. Science. 2019 Aug 9;365(6453). pii: science.aaw5202. doi: 10.1126/science.aaw5202. Epub 2019 Jul 18. 10.1126/science.aaw5202 PubMed 31320556