pYJ07-CRISPR-SOX17-E2-gRNA2
(Plasmid
#131052)
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PurposeSOX17 Knock out
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131052 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneU6-gRNA-spCas9-GFP
- Backbone size w/o insert (bp) 9289
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSOX17 Exon2 gRNA2
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gRNA/shRNA sequenceGTCGGACACCACCGAGGAAA
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SpeciesH. sapiens (human)
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Entrez GeneSOX17 (a.k.a. VUR3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYJ07-CRISPR-SOX17-E2-gRNA2 was a gift from Xiaojun Lian (Addgene plasmid # 131052 ; http://n2t.net/addgene:131052 ; RRID:Addgene_131052)