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pYJ12-PB-U6-ND1-acti-gRNA1
(Plasmid #131056)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131056 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    PB-MS2-sgRNA
  • Backbone size w/o insert (bp) 4131
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NeuroD1 activation gRNA1
  • gRNA/shRNA sequence
    GCGGGGAGGAAGGAGGAGGGG
  • Species
    H. sapiens (human)
  • Entrez Gene
    NEUROD1 (a.k.a. BETA2, BHF-1, MODY6, NEUROD, T2D, bHLHa3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYJ12-PB-U6-ND1-acti-gRNA1 was a gift from Xiaojun Lian (Addgene plasmid # 131056 ; http://n2t.net/addgene:131056 ; RRID:Addgene_131056)