pYJ17-PB-U6-Nkx6.1-acti-gRNA3
(Plasmid
#131061)
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PurposeActivation of Mafa via SAM system
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonePB-MS2-sgRNA
- Backbone size w/o insert (bp) 4131
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNKX6.1 activation gRNA3
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gRNA/shRNA sequenceGGCGCGAGCGGAGAGGCACT
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SpeciesH. sapiens (human)
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Entrez GeneNKX6-1 (a.k.a. NKX6.1, NKX6A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYJ17-PB-U6-Nkx6.1-acti-gRNA3 was a gift from Xiaojun Lian (Addgene plasmid # 131061 ; http://n2t.net/addgene:131061 ; RRID:Addgene_131061)