pYJ35-EZ-Tet-pLKO-shNKX3.1-Puro
(Plasmid
#131078)
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Purposeinducible NKX3-1 knock down
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneEZ-Tet-pLKO-shRNA-Puro
- Backbone size w/o insert (bp) 10633
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Vector typeMammalian Expression, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNKX3.1 shRNA
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gRNA/shRNA sequenceCTAGCGAGACCCAAGTGAAGATATGGTACTAGTCCATATCTTCACTTGGGTCTCTTTTTG
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SpeciesH. sapiens (human)
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Entrez GeneNKX3-1 (a.k.a. BAPX2, NKX3, NKX3.1, NKX3A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYJ35-EZ-Tet-pLKO-shNKX3.1-Puro was a gift from Xiaojun Lian (Addgene plasmid # 131078 ; http://n2t.net/addgene:131078 ; RRID:Addgene_131078)