pYJ36-PB-CRISPR-NKX3.1-E1-gRNA1
(Plasmid
#131079)
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PurposeNKX3-1 knock out
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131079 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonePB-CRISPR
- Backbone size w/o insert (bp) 11659
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Vector typeMammalian Expression, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNKX3.1 Exon1 gRNA1
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gRNA/shRNA sequenceGCTTTCGCCTCCCCGGGCCG
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SpeciesH. sapiens (human)
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Entrez GeneNKX3-1 (a.k.a. BAPX2, NKX3, NKX3.1, NKX3A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYJ36-PB-CRISPR-NKX3.1-E1-gRNA1 was a gift from Xiaojun Lian (Addgene plasmid # 131079 ; http://n2t.net/addgene:131079 ; RRID:Addgene_131079)