pYJ41-AAVS1-XLone-Mafa
(Plasmid
#131084)
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PurposeDonor plasmid of targeted inducible-Mafa knockin
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneAAVS1-XLone donor
- Backbone size w/o insert (bp) 10251
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Vector typeMammalian Expression, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMAFA
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SpeciesH. sapiens (human)
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Insert Size (bp)1062
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Entrez GeneMAFA (a.k.a. INSDM, RIPE3b1, hMafA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains 9xHis in MAFA instead of 10xHis. This difference is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYJ41-AAVS1-XLone-Mafa was a gift from Xiaojun Lian (Addgene plasmid # 131084 ; http://n2t.net/addgene:131084 ; RRID:Addgene_131084)