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pLR05_pGuide_B2M_sgRNA1
(Plasmid #131090)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131090 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGuide
  • Backbone size w/o insert (bp) 3979
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    B2M KO sgRNA 1
  • gRNA/shRNA sequence
    CGCGAGCACAGCTAAGGCCA
  • Species
    H. sapiens (human)
  • Entrez Gene
    B2M (a.k.a. IMD43)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLR05_pGuide_B2M_sgRNA1 was a gift from Xiaojun Lian (Addgene plasmid # 131090 ; http://n2t.net/addgene:131090 ; RRID:Addgene_131090)
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