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phage - ubc - nls - 2xmcp - egfp - BirA*
(Plasmid #131132)


Item Catalog # Description Quantity Price (USD)
Plasmid 131132 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6248
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
  • Insert Size (bp)
  • Mutation
    BirA* = BIrA >R118G
  • Promoter human ubiquitin C (UBC)
  • Tag / Fusion Protein
    • NLS, HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer 5'-ATTACAGGGACAGCAGAGATCC-3'
  • 3′ sequencing primer WPRE-R (5'CATAGCGTAAAAGGAGCAACA-3')
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phage - ubc - nls - 2xmcp - egfp - BirA* was a gift from Ralf Jansen (Addgene plasmid # 131132 ; ; RRID:Addgene_131132)
  • For your References section:

    beta-Actin mRNA interactome mapping by proximity biotinylation. Mukherjee J, Hermesh O, Eliscovich C, Nalpas N, Franz-Wachtel M, Macek B, Jansen RP. Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):12863-12872. doi: 10.1073/pnas.1820737116. Epub 2019 Jun 12. 10.1073/pnas.1820737116 PubMed 31189591