Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)
(Kit #
1000000215
)
Depositing Lab: Adam Cohen
Plasmids and entry vectors for diverse fluorescent sensors of physiological parameters in cells.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE. Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. PubMed PMID: 32753572
Description
The Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC) plasmid kit is comprised of 19 lentiviral plasmids for expression of diverse fluorescent sensors of physiological parameters in cells and 16 entry vectors for cloning applications.
The following sensors are included in this kit:
- Plasmid membrane voltage sensor (QuasAr2-mOrange2) and channelrhodopsin (CheRiff-eGFP)
- Cytosolic, mitochondrial, and endoplasmid reticulum calcium sensors (GCaMP6F, Mitycam, and GCaMPer), superecliptic pHIuorins, ratiometric pHIuorins, and glutathione redox sensors (Grx1-roGFP2, mito-roGFP2-Grx1, and Grx1-roGFP1-ie_ER)
- Cytosolic chloride (ClopHensorN), Peredox NADH/NAD+, and PercevalHR ATP/ADP sensors
- Cytosolic (roGFP2-Orp1) and mitochondrial (mito-roGFP2-Orp1) oxidation sensors
Array of islands of HEK cells, each expressing a fluorescent reporter of a distinct physiological modality.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC) was a gift from Adam Cohen (Addgene kit #1000000175).”
For your Reference section:
Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE. Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. PubMed PMID: 32753572
Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC) - #1000000175
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin | |
| Kanamycin |