MigR1-Flag-TRIB2
(Plasmid #131154)

• Purpose: Retroviral expression of flag-tagged mouse TRIB2
• Depositing Labs: Karen Keeshan, Warren Pear
• Publication: Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi: 10.1016/j.ccr.2006.09.012.

Backbone

• Vector backbone: MIGR1
• Backbone manufacturer: Warren Pear (Addgene plasmid # 27490)
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trib2
• Species: M. musculus (mouse)
• Insert Size (bp): 1032
• Entrez Gene: Trib2 (a.k.a. AW319517, TRB, TRB-2, TRB2)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Murine Trib2 (NM_144551.5); XhoI-ATG-Flag-Trib2-EcoRI