pGEM-PH-citrine
(Plasmid
#131406)
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PurposePurpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine
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Depositing Lab
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Depositing OrganizationEuropean Molecular Biology Laboratory (EMBL), Heidelberg
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131406 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 131406-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 131406-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGEMHE
- Total vector size (bp) 4356
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Vector typein vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePH
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Alt namePLCD1 (PH domain of the human phospholipase C delta 1)
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SpeciesH. sapiens (human)
- Promoter T7
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Tag
/ Fusion Protein
- citrine yellow fluorescent protein (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Alex McDougall; pRN3-PH-GFP described in doi:10.1242/jcs.00846.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 131406-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 131406-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-PH-citrine was a gift from Lars Hufnagel (Addgene plasmid # 131406 ; http://n2t.net/addgene:131406 ; RRID:Addgene_131406) -
For your References section:
Contact area-dependent cell communication and the morphological invariance of ascidian embryogenesis. Guignard L, Fiuza UM, Leggio B, Laussu J, Faure E, Michelin G, Biasuz K, Hufnagel L, Malandain G, Godin C, Lemaire P. Science. 2020 Jul 10;369(6500). pii: 369/6500/eaar5663. doi: 10.1126/science.aar5663. 10.1126/science.aar5663 PubMed 32646972
