EP-Pol vector
(Plasmid
#131686)
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PurposeA lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131686 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRRL-cPPT-X-PRE-SIN
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAdenovirus DNA polymerase
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Alt nameE2B DNA polymerase
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SpeciesAdenovirus type 5
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MutationF421Y, D827A
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EP-Pol vector was a gift from Matthew D. Shoulders (Addgene plasmid # 131686 ; http://n2t.net/addgene:131686 ; RRID:Addgene_131686) -
For your References section:
An Adaptable Platform for Directed Evolution in Human Cells. Berman CM, Papa LJ 3rd, Hendel SJ, Moore CL, Suen PH, Weickhardt AF, Doan ND, Kumar CM, Uil TG, Butty VL, Hoeben RC, Shoulders MD. J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14. 10.1021/jacs.8b10937 PubMed 30427676
Map uploaded by the depositor.
