pUC-AdEvolve-DEST
(Plasmid #131692)

• Purpose: (Empty Backbone) A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.
• Depositing Lab: Matthew D. Shoulders
• Publication: Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14.

Backbone

• Vector backbone: pAd⁄CMV⁄V5-DEST
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size (bp): 36831
• Modifications to backbone: The orientation of the gateway cassette is reversed and a kanamycin resistance gene is inserted into the gateway cassette. The polymerase and protease genes are deleted. The mCherry fluorescent marker is inserted near the E4 region.
• Vector type: Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B gyrA462
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • pUC-AdEvolve-DEST.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.