Wild-type AdPol vector
(Plasmid #131685)

• Purpose: A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase
• Depositing Lab: Matthew D. Shoulders
• Publication: Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14.

Backbone

• Vector backbone: pRRL-cPPT-X-PRE-SIN
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Adenovirus DNA polymerase
• Alt name: E2B DNA polymerase
• Species: Adenovirus type 5
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Resource Information

• Supplemental Documents:
  • Wild-type AdPol vector.gb
• A portion of this plasmid was derived from a plasmid made by: Dr. Robert C. Hoeben, Leiden University Medical Center, Leiden, The Netherlands

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Uil, T. G.; Vellinga, J.; de Vrij, J.; van den Hengel, S. K.; Rabelink, M. J. W. E.; Cramer, S. J.; Eekels, J. J. M.; Ariyurek, Y.; van Galen, M.; Hoeben, R. C. Directed adenovirus evolution using engineered mutator viral polymerases. Nucleic Acids Res. 2011, 39, e30.