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Addgene

pIRES2-DsRed-MST-Kv2.1
(Plasmid #131707)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131707 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES2-DsRed-Express
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 7900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNB1
  • Alt name
    potassium voltage-gated channel subfamily B member 1
  • Alt name
    Kv2.1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2577
  • GenBank ID
    NM_004975
  • Entrez Gene
    KCNB1 (a.k.a. DRK1, Kv2.1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer CMV_fwd_primer
  • 3′ sequencing primer dsRed1_N_primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES2-DsRed-MST-Kv2.1 was a gift from Jennifer Kearney (Addgene plasmid # 131707 ; http://n2t.net/addgene:131707 ; RRID:Addgene_131707)
  • For your References section:

    Voltage-gated potassium channel KCNV2 (Kv8.2) contributes to epilepsy susceptibility. Jorge BS, Campbell CM, Miller AR, Rutter ED, Gurnett CA, Vanoye CG, George AL Jr, Kearney JA. Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5443-8. doi: 10.1073/pnas.1017539108. Epub 2011 Mar 14. 10.1073/pnas.1017539108 PubMed 21402906