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PurposeFRET-based sensor for CaMKII activity
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Amherst
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 132415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 132415-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 132415-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDNA3.1
- Backbone size w/o insert (bp) 5475
- Total vector size (bp) 7476
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRESCA-syntide
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SpeciesH. sapiens (human)
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Insert Size (bp)2001
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GATCCGGCGGCGCCGGCGGCGGCccgctggcgcgcgccctgaccgtggcgggcctgccgggcaaaaaaGGC
- 3′ sequencing primer GGCCGCCttttttgcccggcaggcccgccacggtcagggcgcgcgccagcggGCCGCCGCCGGCGCCGCCG
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMegan West, UMass-Amherst
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 132415-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 132415-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FRESCA-syntide was a gift from Margaret Stratton (Addgene plasmid # 132415 ; http://n2t.net/addgene:132415 ; RRID:Addgene_132415) -
For your References section:
FRET-based sensor for CaMKII activity (FRESCA): A useful tool for assessing CaMKII activity in response to Ca(2+) oscillations in live cells. Ardestani G, West MC, Maresca TJ, Fissore RA, Stratton MM. J Biol Chem. 2019 Jun 14. pii: RA119.009235. doi: 10.1074/jbc.RA119.009235. 10.1074/jbc.RA119.009235 PubMed 31201271
Map uploaded by the depositor.
