Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1325||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepRS303 (based on pBluescript
- Backbone size w/o insert (bp) 5000
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Gene/Insert nameGPD promoter-HSP82
Alt nameYeast Heat shock protein 82
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)3800
MutationWild type gene
Entrez GeneHSP82 (a.k.a. YPL240C, HSP90)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1, BamH1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid maintained in DH5alpha.
ClaI-NotI fragment from pTGpd/P82 was cloned into pRS303
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:piHGpd/P82 was a gift from Susan Lindquist (Addgene plasmid # 1325 ; http://n2t.net/addgene:1325 ; RRID:Addgene_1325)
For your References section:In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone. Nathan DF, Vos MH, Lindquist S. Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12949-56. 10.1073/pnas.94.24.12949 PubMed 9371781