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Methyl-binding domain protein 2 (MBD2) coiled-coil domain
(Plasmid #132721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132721 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET15b
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Methyl-binding protein domain 2 coiled-coil domain
  • Alt name
    MBD2
  • Species
    Synthetic
  • Insert Size (bp)
    204
  • Promoter T/
  • Tag / Fusion Protein
    • N-terminal His-tag. Flanking linkers to match accompanying biosensor (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Fwd
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Purchased as synthetic gene from Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Methyl-binding domain protein 2 (MBD2) coiled-coil domain was a gift from Magnus Kjaergaard (Addgene plasmid # 132721 ; http://n2t.net/addgene:132721 ; RRID:Addgene_132721)

  • For your References section:

    Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers. Sorensen CS, Kjaergaard M. Methods Mol Biol. 2020;2141:505-518. doi: 10.1007/978-1-0716-0524-0_25. 10.1007/978-1-0716-0524-0_25 PubMed 32696374
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