pcDNA GFP10-RHOA
(Plasmid #133068)

• Purpose: Expresses RHOA GTPase with tripartite split GFP10
• Depositing Lab: Stéphanie Cabantous
• Publication: Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.210419. doi: 10.1242/jcs.210419.

Backbone

• Vector backbone: pcDNA 3.1
• Total vector size (bp): 5695
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ras homolog family member A
• Species: H. sapiens (human)
• GenBank ID: NM 001313944
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP10 (split-GFP tripartite) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: ClaI (unknown if destroyed)
• 5′ sequencing primer: CMV_F
• 3′ sequencing primer: CAAACAACAGATGGCTGGC

Resource Information

• Supplemental Documents:
  • pcdna3 GFP10-RhoA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry