High-content tripartite split-GFP cell-based assays to screen for modulators of small GTPase activation.
Koraichi F, Gence R, Bouchenot C, Grosjean S, Lajoie-Mazenc I, Favre G, Cabantous S
J Cell Sci. 2018 Jan 8;131(1). pii: jcs.210419. doi: 10.1242/jcs.210419. PubMed Article
Koraichi F, Gence R, Bouchenot C, Grosjean S, Lajoie-Mazenc I, Favre G, Cabantous S
J Cell Sci. 2018 Jan 8;131(1). pii: jcs.210419. doi: 10.1242/jcs.210419. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130271 | pTRIP CMV GFP1-9 | Lentiviral expression vector for stable expression of GFP1-9 |
| 133068 | pcDNA GFP10-RHOA | Expresses RHOA GTPase with tripartite split GFP10 |
| 133069 | pcDNA RBD-GFP11 | Expresses RBD domain with tripartite split GFP11 (M4) |
| 182231 | pTRIP TRE-BI GFP10-RHOB/RBD-GFP11 | Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain |
| 182232 | pTRIP TRE-BI GFP10-RHOA/RBD-GFP11 | Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain |
| 182236 | pTRIP CMV VHHG4 | Expresses GFP nanobody for stable expression in mammalian cells |
| 182237 | pcDNA GFP10-RHOB | Expresses RHOB GTPase with tripartite split GFP10 tag |