Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

High-content tripartite split-GFP cell-based assays to screen for modulators of small GTPase activation.
Koraichi F, Gence R, Bouchenot C, Grosjean S, Lajoie-Mazenc I, Favre G, Cabantous S
J Cell Sci. 2018 Jan 8;131(1). pii: jcs.210419. doi: 10.1242/jcs.210419.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
130271pTRIP CMV GFP1-9Lentiviral expression vector for stable expression of GFP1-9
133068pcDNA GFP10-RHOAExpresses RHOA GTPase with tripartite split GFP10
133069pcDNA RBD-GFP11Expresses RBD domain with tripartite split GFP11 (M4)
182231pTRIP TRE-BI GFP10-RHOB/RBD-GFP11Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain
182232pTRIP TRE-BI GFP10-RHOA/RBD-GFP11Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain
182236pTRIP CMV VHHG4Expresses GFP nanobody for stable expression in mammalian cells
182237pcDNA GFP10-RHOBExpresses RHOB GTPase with tripartite split GFP10 tag

Recombinant Antibodies from Article