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pJH548
(Plasmid #13328)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13328 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 13328-D50 50 μg of DNA in Tris buffer $495
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DNA 13328-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCDM8
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4356
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Grow in SupF strain. The supF tRNA suppressor gene is used for selection in E. coli.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAG-1
  • Alt name
    RAG1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Rag1 (a.k.a. Rag-1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA was synthesized by reverse transcription of total RNA obtained from cell line 22D6, an Abelson murine leukemia virus transformed pre-B cell line. RAG-1 cDNA was then amplified by PCR, using specific primers designed also to add restriction enzyme recognition sites adjacent to the largest open reading frame for subsequent cloning. PCR products were subcloned into the pCDM8 vector.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH548 was a gift from Martin Gellert (Addgene plasmid # 13328 ; http://n2t.net/addgene:13328 ; RRID:Addgene_13328)

  • For your References section:

    Expression and V(D)J recombination activity of mutated RAG-1 proteins. Sadofsky MJ, Hesse JE, McBlane JF, Gellert M. Nucleic Acids Res. 1993 Dec 11. 21(24):5644-50. 10.1093/nar/21.24.5644 PubMed 8284210
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