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(Plasmid #133281)


Item Catalog # Description Quantity Price (USD)
Plasmid 133281 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6894
  • Total vector size (bp) 7850
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)
  • Promoter CMV
  • Tag / Fusion Protein
    • Biotin Acceptor Peptide contains 7-His-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CTCTGGCTAACTAGAGAAC
  • 3′ sequencing primer AGACGGCAATATGGTGGA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

1. Kulyyassov, A. et al. PUB-MS: A Mass Spectrometry-based Method to Monitor Protein-Protein Proximity in vivo. Journal of Proteome Research10, 4416-4427, doi:10.1021/pr200189p (2011)
2. Shoaib, M. et al. PUB-NChIP-"in vivo biotinylation" approach to study chromatin in proximity to a protein of interest. Genome Research23, 331-340, doi:10.1101/gr.134874.111 (2013)..

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-BAP-Sox2 was a gift from Arman Kulyyassov (Addgene plasmid # 133281 ; ; RRID:Addgene_133281)
  • For your References section:

    In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2 and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation. Kulyyassov A, Ogryzko V. Biomolecules. 2020 Jan 16;10(1). pii: biom10010142. doi: 10.3390/biom10010142. 10.3390/biom10010142 PubMed 31963153