Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pXGFPC-5 Pxyl-dcas9 (Sth3)
(Plasmid #133317)

  • Purpose
    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance
  • Depositing Lab
  • Sequence Information

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 133317 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pXGFPC-5
  • Backbone size w/o insert (bp) 6296
  • Total vector size (bp) 10463
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dcas9 (Streptococcus thermophilus #3)
  • Species
    Streptococcus thermophilus
  • Insert Size (bp)
    4167
  • Promoter xylose

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGGTCAGACAACCTACTTGCCGTC
  • 3′ sequencing primer TCGGCTGCGGCGAGCGGTATCA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Jeremy M. Rock "Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform" Nature microbiology 2017
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXGFPC-5 Pxyl-dcas9 (Sth3) was a gift from Michael Laub (Addgene plasmid # 133317 ; http://n2t.net/addgene:133317 ; RRID:Addgene_133317)
  • For your References section:

    A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus. Guzzo M, Castro LK, Reisch CR, Guo MS, Laub MT.. mBio 10.1128/mBio.02415-19