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A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus
Guzzo M, Castro LK, Reisch CR, Guo MS, Laub MT.
mBio
Article

Plasmids from Article

ID Plasmid Purpose  
133316pXGFPC-5 Pxyl-dcas9 (Spy)expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
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133317pXGFPC-5 Pxyl-dcas9 (Sth3)expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance
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133318pXGFPC-5 Pxyl-dcas9 (Spa)expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
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133319pVCERC-5 Pvan-dcas9 (Sth3)expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance
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133334pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrAfor constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus
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133339pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrAfor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus
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133340pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus
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133341pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus
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133342pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrAfor constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus
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133343pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus
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133344pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaAfor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus
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133345pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaAfor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus
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133347pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaAexpression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
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133348pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaAexpression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)
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