A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus.
Guzzo M, Castro LK, Reisch CR, Guo MS, Laub MT
mBio. 2020 Jan 14;11(1):e02415-19. doi: 10.1128/mBio.02415-19. PubMed Article
Guzzo M, Castro LK, Reisch CR, Guo MS, Laub MT
mBio. 2020 Jan 14;11(1):e02415-19. doi: 10.1128/mBio.02415-19. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133316 | pXGFPC-5 Pxyl-dcas9 (Spy) | expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance |
| 133317 | pXGFPC-5 Pxyl-dcas9 (Sth3) | expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance |
| 133318 | pXGFPC-5 Pxyl-dcas9 (Spa) | expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance |
| 133319 | pVCERC-5 Pvan-dcas9 (Sth3) | expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance |
| 133334 | pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA | for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus |
| 133339 | pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA | for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus |
| 133340 | pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1 | for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus |
| 133341 | pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2 | for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus |
| 133342 | pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA | for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus |
| 133343 | pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3 | for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus |
| 133344 | pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA | for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus |
| 133345 | pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA | for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus |
| 133347 | pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA | expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus) |
| 133348 | pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA | expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus) |