pVCERC-5 Pvan-dcas9 (Sth3)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||133319||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5431
- Total vector size (bp) 9598
Growth in Bacteria
Gene/Insert namedcas9 (Streptococcus thermophilus #3)
Insert Size (bp)4167
- Promoter vanillate
- Cloning method Gibson Cloning
- 5′ sequencing primer CTGGACTCTAGCCGACCGACT
- 3′ sequencing primer ctgataccgctggcgaaagg (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pVCERC-5 Pvan-dcas9 (Sth3) was a gift from Michael Laub (Addgene plasmid # 133319 ; http://n2t.net/addgene:133319 ; RRID:Addgene_133319)
For your References section:A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus. Guzzo M, Castro LK, Reisch CR, Guo MS, Laub MT.. mBio 10.1128/mBio.02415-19