PurposeFluorescent reporter for calcium imaging and photoactivation.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||133412||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4632
- Total vector size (bp) 6777
Vector typeMammalian Expression, AAV
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)2145
- Promoter CAG
- Cloning method Gibson Cloning
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer EBV Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pAAV2 : The Hope Center Viral Vectors Core at Washington University in St. Louis
GCaMP6f :pGP-CMV-GCaMP6f was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 40755 ; http://n2t.net/addgene:40755 ; RRID:Addgene_40755)
PAmCherry: pPAmCherry1-C1 was a gift from Vladislav Verkhusha (Addgene plasmid # 31929 ; http://n2t.net/addgene:31929 ; RRID:Addgene_31929)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV2-GCaMP6f-P2A-PAmCherry was a gift from Tim Holy (Addgene plasmid # 133412 ; http://n2t.net/addgene:133412 ; RRID:Addgene_133412)
For your References section:Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types. Lee D, Kume M, Holy TE. Science. 2019 Dec 13;366(6471):1384-1389. doi: 10.1126/science.aax8055. 10.1126/science.aax8055 PubMed 31831669