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(Plasmid #133732)


Item Catalog # Description Quantity Price (USD)
Plasmid 133732 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Vector Bioloabs
  • Backbone size w/o insert (bp) 4685
  • Total vector size (bp) 6251
  • Modifications to backbone
    The inserts containing the pTRE3G-Bi-gePSI (α-chain and β-chain-ODC36) with SV40 poly(A) and the pCamk2a-AcGFP1 were inserted between the AAV2 ITRs.
  • Vector type
    Mammalian Expression, AAV
  • Selectable markers
    No selectable marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
    Zea mays
  • Insert Size (bp)
  • Promoter pTRE3G-Bi
  • Tag / Fusion Protein
    • murine ornithine decarboxylase - degron (ODC36) (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Species
    Zea mays
  • Insert Size (bp)
  • Promoter pTRE3G-Bi

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
  • Species
    Aequorea coerulescens
  • Insert Size (bp)
  • Promoter pCamk2a

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE3G-Bi-gePSI-pCamk2a-AcGFP was a gift from Erin Schuman (Addgene plasmid # 133732 ; ; RRID:Addgene_133732)
  • For your References section:

    A genetically encodable cell-type-specific protein synthesis inhibitor. Heumuller M, Glock C, Rangaraju V, Biever A, Schuman EM. Nat Methods. 2019 Jul 15. pii: 10.1038/s41592-019-0468-x. doi: 10.1038/s41592-019-0468-x. 10.1038/s41592-019-0468-x PubMed 31308551