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A genetically encodable cell-type-specific protein synthesis inhibitor.
Heumuller M, Glock C, Rangaraju V, Biever A, Schuman EM
Nat Methods. 2019 Jul 15. pii: 10.1038/s41592-019-0468-x. doi: 10.1038/s41592-019-0468-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
131008pTRE3G-Bi-gePSI-pCMV-AcGFPExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.
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133727pCAMKIIa-TetOn3GPlasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter.
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133728pGFAP-TetOn3GPlasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter.
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133729pCMV-FLEx-TetOn3G-3xHA-FLExPlasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.
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133730pTRE3G-Bi-α-chainExpresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.
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133731pTRE3G-Bi-β-chainExpresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.
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133732pTRE3G-Bi-gePSI-pCamk2a-AcGFPExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.
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133733pTRE3G-Bi-gePSI-pGFAP-AcGFPExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.
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