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(Plasmid #133750)


Item Catalog # Description Quantity Price (USD)
Plasmid 133750 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 10455
  • Total vector size (bp) 14088
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    Mutant P667A EGFR-HA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    proline 667 converted to alanine (Please see depositor comments below)
  • GenBank ID
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA)
  • Promoter EF1-alpha
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MLU1 (unknown if destroyed)
  • 3′ cloning site SPE1 (unknown if destroyed)
  • 5′ sequencing primer CAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer GCGTAAAAGGAGCAACATAG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mutation in this human EGFR cDNA corresponds to the conversion of proline 667 to alanine described in Cheng He et al., JBC, 2002. In this larger isoform of human EGFR the proline is located at 691. The site of the mutation is 685-RELVEP-691.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PWPXLD-EGFR-P667A was a gift from Chay Kuo (Addgene plasmid # 133750 ; ; RRID:Addgene_133750)
  • For your References section:

    EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation. Abdi K, Neves G, Pyun J, Kiziltug E, Ahrens A, Kuo CT. Cell Rep. 2019 Aug 20;28(8):2012-2022.e4. doi: 10.1016/j.celrep.2019.07.056. 10.1016/j.celrep.2019.07.056 PubMed 31433979