PurposeEGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||133750||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10455
- Total vector size (bp) 14088
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Gene/Insert nameMutant P667A EGFR-HA
SpeciesH. sapiens (human)
Insert Size (bp)3633
Mutationproline 667 converted to alanine (Please see depositor comments below)
Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA)
- Promoter EF1-alpha
/ Fusion Protein
- HA tag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site MLU1 (unknown if destroyed)
- 3′ cloning site SPE1 (unknown if destroyed)
- 5′ sequencing primer CAAGCCTCAGACAGTGGTTC
- 3′ sequencing primer GCGTAAAAGGAGCAACATAG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The mutation in this human EGFR cDNA corresponds to the conversion of proline 667 to alanine described in Cheng He et al., JBC, 2002. In this larger isoform of human EGFR the proline is located at 691. The site of the mutation is 685-RELVEP-691.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PWPXLD-EGFR-P667A was a gift from Chay Kuo (Addgene plasmid # 133750 ; http://n2t.net/addgene:133750 ; RRID:Addgene_133750)
For your References section:EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation. Abdi K, Neves G, Pyun J, Kiziltug E, Ahrens A, Kuo CT. Cell Rep. 2019 Aug 20;28(8):2012-2022.e4. doi: 10.1016/j.celrep.2019.07.056. 10.1016/j.celrep.2019.07.056 PubMed 31433979