PurposeExpresses human codon-optimized intein-Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||133782||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeInsect Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameHuman codon-optimized intein-Cas9
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
HMS clone ID number: EvNO00483430.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMK33/intein-Cas9_S219-3XFLAG was a gift from Norbert Perrimon (Addgene plasmid # 133782 ; http://n2t.net/addgene:133782 ; RRID:Addgene_133782)
For your References section:Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells. Viswanatha R, Li Z, Hu Y, Perrimon N. Elife. 2018 Jul 27;7. pii: 36333. doi: 10.7554/eLife.36333. 10.7554/eLife.36333 PubMed 30051818