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Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells.
Viswanatha R, Li Z, Hu Y, Perrimon N
Elife. 2018 Jul 27;7. pii: 36333. doi: 10.7554/eLife.36333.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
133781pMK33/Cas9Expresses drosophila-optimized Cas9
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133782pMK33/intein-Cas9_S219-3XFLAGExpresses human codon-optimized intein-Cas9
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133783pLib6.4Library backbone
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134582Drosophila_sgRNA_group_1Targets kinases, phosphatases, and Drosophila orthologs of drug targets
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134583Drosophila_sgRNA_group_2Targets Drosophila orthologs of vertebrate genes that have undergone expansion in the vertebrate line
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134584Drosophila_sgRNA_group_3Targets all other Drosophila genes not included in Groups 1 and 2
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