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pMK33/Cas9
(Plasmid #133781)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 133781 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMK33
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drosophila-optimized Cas9

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: EvNO00483429.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMK33/Cas9 was a gift from Norbert Perrimon (Addgene plasmid # 133781 ; http://n2t.net/addgene:133781 ; RRID:Addgene_133781)
  • For your References section:

    Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells. Viswanatha R, Li Z, Hu Y, Perrimon N. Elife. 2018 Jul 27;7. pii: 36333. doi: 10.7554/eLife.36333. 10.7554/eLife.36333 PubMed 30051818