Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMK33/Cas9
(Plasmid #133781)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 133781 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drosophila-optimized Cas9

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

HMS clone ID number: EvNO00483429.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMK33/Cas9 was a gift from Norbert Perrimon (Addgene plasmid # 133781 ; http://n2t.net/addgene:133781 ; RRID:Addgene_133781)
  • For your References section:

    Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells. Viswanatha R, Li Z, Hu Y, Perrimon N. Elife. 2018 Jul 27;7. pii: 36333. doi: 10.7554/eLife.36333. 10.7554/eLife.36333 PubMed 30051818