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pET23a RanGAP
(Plasmid #13387)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13387 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET23a(+)
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 3700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RanGAP
  • Alt name
    RanGAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Entrez Gene
    RANGAP1 (a.k.a. Fug1, RANGAP, SD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    V. Gerke, University of Muenster, Germany
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a RanGAP was a gift from Mary Dasso (Addgene plasmid # 13387 ; http://n2t.net/addgene:13387 ; RRID:Addgene_13387)
  • For your References section:

    SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. Joseph J, Tan SH, Karpova TS, McNally JG, Dasso M. J Cell Biol. 2002 Feb 18. 156(4):595-602. 10.1083/jcb.200110109 PubMed 11854305