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pGEX-T-rnVAPA-1-125
(Plasmid #13396)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13396 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VAP-A
  • Alt name
    VAPA
  • Alt name
    VAP
  • Alt name
    VAP-33
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    380
  • Entrez Gene
    Vapa
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

ncbi gi:118142811 / swiss-prot entry Q9Z270

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-T-rnVAPA-1-125 was a gift from Axel Brunger (Addgene plasmid # 13396 ; http://n2t.net/addgene:13396 ; RRID:Addgene_13396)
  • For your References section:

    Structural basis of FFAT motif-mediated ER targeting. Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT. Structure. 2005 Jul . 13(7):1035-45. 10.1016/j.str.2005.04.010 PubMed 16004875