|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||133967||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerYuhui Sun
Vector typeCRISPR, Synthetic Biology
Selectable markersApramycin for Streptomyces selection
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Gibson Cloning
Please visit https://www.biorxiv.org/content/10.1101/630137v1.full for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSCBE3-HF was a gift from Yuhui Sun (Addgene plasmid # 133967 ; http://n2t.net/addgene:133967 ; RRID:Addgene_133967)
For your References section:Base editing in Streptomyces with Cas9-deaminase fusions. Zhong Z, Guo J, Deng L, Chen L, Wang J, Li S, Xu W, Deng Z, Sun Y. BioRxiv 630137 10.1101/630137