IF189
(Bacterial strain
#134836)
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PurposeRapid and efficient recombineering and purificiation of modified lambda phage DNA
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 134836 | Bacteria in agar stab | 1 | $85 * | |
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Backbone
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Vector backbonenone
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature30°C
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Growth Strain(s)IF189
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Growth instructionsGrow at 30°C, but restricted at 42°C due to the induction of phage particles and cell lysis
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Copy numberUnknown
Gene/Insert
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Gene/Insert namelysogen of a temperature-inducible bacteriophage lambda
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genotype is [λ+ Lac- galK2 IN(rrnD-rrnE)1 rph-1], a derivative of strain W3102
Phenotypic assay: Grow at 30°C, but restricted at 42°C due to the induction of phage particles and cell lysis.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IF189 was a gift from IIya Finkelstein (Addgene plasmid # 134836) -
For your References section:
Efficient modification of lambda-DNA substrates for single-molecule studies. Kim Y, de la Torre A, Leal AA, Finkelstein IJ. Sci Rep. 2017 May 18;7(1):2071. doi: 10.1038/s41598-017-01984-x. 10.1038/s41598-017-01984-x PubMed 28522818